JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.

ABSTRACT:

Background

Human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) hold great promise for cardiac disease modelling, drug discovery and regenerative medicine. Despite the advancement in various differentiation protocols, the heterogeneity of the generated population composed of diverse cardiac subtypes poses a significant challenge to their practical applications. Mixed populations of cardiac subtypes can compromise disease modelling and drug discovery, while transplanting them may lead to undesired arrhythmias as they may not integrate and synchronize with the host tissue's contractility. It is therefore crucial to identify cell surface markers that could enable high purity of ventricular CMs for subsequent applications.

Methods

By exploiting the fact that immature

SUBMITTER: Liew LC 

PROVIDER: S-EPMC10720068 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature