Wilforine inhibits rheumatoid arthritis pathology through the Wnt11/β-catenin signaling pathway axis.

ABSTRACT:

Background

Wilforine (WFR) is a monomeric compound of the anti-RA plant Tripterygium wilfordii Hook. f. (TwHF). Whether WFR has anti-RA effect, its molecular mechanism has not been elucidated.

Aim of the study

Our study aims to clarify how WFR inhibits fibroblast-like synovial cells (FLS) activation and improves RA through Wnt11 action on the Wnt11/β-catenin signaling pathway.

Methods

The therapeutic effect of WFR on collagen-induced arthritis (CIA) rats was evaluated using methods such as rat arthritis score. The inhibitory effects and signaling pathways of WFR on the proliferation and inflammatory response of CIA FLS and RA FLS were studied using ELISA, CCK-8, RT-qPCR, Western blot, and immunofluorescence methods.

Results

WFR could effectively alleviate the a

SUBMITTER: Huang Y 

PROVIDER: S-EPMC10720104 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature