Generation of a bank of clinical-grade, HLA-homozygous iPSC lines with high coverage of the Spanish population.

ABSTRACT:

Background

Induced pluripotent stem cell (iPSC)-derived cell therapies are an interesting new area in the field of regenerative medicine. One of the approaches to decrease the costs of iPSC-derived therapies is the use of allogenic homozygous human leukocyte antigen (HLA)-matched donors to generate iPSC lines and to build a clinical-grade iPSC bank covering a high percentage of the Spanish population.

Methods

The Spanish Stem Cell Transplantation Registry was screened for cord blood units (CBUs) homozygous for the most common HLA-A, HLA-B and HLA-DRB1 haplotypes. Seven donors were selected with haplotypes covering 21.37% of the haplotypes of the Spanish population. CD34-positive hematopoietic progenitors were isolated from the mononuclear cell fraction of frozen cord blood u

SUBMITTER: Kuebler B 

PROVIDER: S-EPMC10720139 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature