Proteomic profiling of human menisci from mild joint degeneration and end-stage osteoarthritis versus healthy controls.

ABSTRACT:

Objective

To gain new insight into the molecular changes of the meniscus by comparing the proteome profiles of healthy controls with mild degeneration and end-stage osteoarthritis (OA).

Method

We obtained tissue plugs from lateral and medial menisci of 37 individuals (central part of the posterior horn) classified as healthy (n ​= ​12), mild signs of joint damage (n ​= ​13) and end-stage OA (n ​= ​12). The protein profile was analysed by nano-liquid chromatography-mass spectrometry using data-independent acquisition and quantified by Spectronaut. Linear-mixed effects modelling was applied to extract the between-group comparisons.

Results

A similar protein profile was observed for the mild group as compared to healthy controls while the most different group was end-sta

SUBMITTER: Paz-Gonzalez R 

PROVIDER: S-EPMC10720269 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature