Analyzing the cellular plasma membrane by fast and efficient correlative STED and platinum replica EM.

ABSTRACT: The plasma membrane of mammalian cells links transmembrane receptors, various structural components, and membrane-binding proteins to subcellular processes, allowing inter- and intracellular communication. Therefore, membrane-binding proteins, together with structural components such as actin filaments, modulate the cell membrane in their flexibility, stiffness, and curvature. Investigating membrane components and curvature in cells remains challenging due to the diffraction limit in light microscopy. Preparation of 5-15-nm-thin plasma membrane sheets and subsequent inspection by metal replica transmission electron microscopy (TEM) reveal detailed information about the cellular membrane topology, including the structure and curvature. However, electron microscopy cannot identify proteins a

SUBMITTER: Puchkov D 

PROVIDER: S-EPMC10720448 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature