Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance.

ABSTRACT: We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-¹³C]fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conducti

SUBMITTER: Eills J 

PROVIDER: S-EPMC10720634 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature