A disruptive clickable antibody design for the generation of antibody-drug conjugates.

ABSTRACT:

Background

Antibody-drug conjugates are cancer therapeutics that combine specificity and toxicity. A highly cytotoxic drug is covalently attached to an antibody that directs it to cancer cells. The conjugation of the drug-linker to the antibody is a key point in research and development as well as in industrial production. The consensus is to conjugate the drug to a surface-exposed part of the antibody to ensure maximum conjugation efficiency. However, the hydrophobic nature of the majority of drugs used in antibody-drug conjugates leads to an increased hydrophobicity of the generated antibody-drug conjugates, resulting in higher liver clearance and decreased stability.

Methods

In contrast, we describe a non-conventional approach in which the drug is conjugated in a buried p

SUBMITTER: Rakotoarinoro N 

PROVIDER: S-EPMC10720948 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature