UPLC-MS/MS method for quantitative determination of the advanced glycation endproducts N^ε-(carboxymethyl)lysine and N^ε-(carboxyethyl)lysine.

ABSTRACT: During blood storage, red blood cells (RBCs) undergo physical, chemical, and metabolic changes that may contribute to post-transfusion complications. Due to the hyperglycemic environment of typical solutions used for RBC storage, the formation of advanced glycation endproducts (AGEs) on the stored RBCs has been implicated as a detrimental chemical change during storage. Unfortunately, there are limited studies involving quantitative determination and differentiation of carboxymethyl-lysine (CML) and carboxyethyl-lysine (CEL), two commonly formed AGEs, and no reported studies comparing these AGEs in experimental storage solutions. In this study, CML and CEL were identified and quantified on freshly drawn blood samples in two types of storage solutions, standard additive solution 1 (AS-1) an

SUBMITTER: Skrajewski-Schuler LA 

PROVIDER: S-EPMC10720951 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature