Laser Capture Proteomics: spatial tissue molecular profiling from the bench to personalized medicine.

ABSTRACT:

Introduction

Laser Capture Microdissection (LCM) uses a laser to isolate, or capture, specific cells of interest in a complex heterogeneous tissue section, under direct microscopic visualization. Recently, there has been a surge of publications using LCM for tissue spatial molecular profiling relevant to a wide range of research topics.

Areas covered

We summarize the many advances in tissue Laser Capture Proteomics (LCP) using mass spectrometry for discovery, and protein arrays for signal pathway network mapping. This review emphasizes: a) transition of LCM phosphoproteomics from the lab to the clinic for individualized cancer therapy, and b) the emerging frontier of LCM single cell molecular analysis combining proteomics with genomic, and transcriptomic analysis. The search

SUBMITTER: Liotta LA 

PROVIDER: S-EPMC10720974 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature