Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B.

ABSTRACT: Capillary zone electrophoresis (CZE) is a powerful tool for high resolution chemical separations. Applying CZE to microbial samples may facilitate a deeper understanding of bacterial physiology and behavior. However, the study of complex microbial samples has been limited by the uncontrolled hetero-aggregation of bacterial cells under an applied electric field. We tested a wide range of sample buffers and buffer additives for the optimization of bacterial CZE separations using a 20 mM Tris-HCl background electrolyte. By modifying the sample buffer, but not the background electrolyte, we retain constant separation conditions, which aids in the comparison of the sample buffer additives. We report optimized methods for automated CZE separation and simultaneous fractionation of Escherichia col

SUBMITTER: Huge BJ 

PROVIDER: S-EPMC10721931 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature