Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells.

ABSTRACT: The Gal4/UAS system is a versatile tool to manipulate exogenous gene expression of cells spatially and temporally in many model organisms. Many variations of light-controllable Gal4/UAS system are now available, following the development of photo-activatable (PA) molecular switches and integration of these tools. However, many PA-Gal4 transcription factors have undesired background transcription activities even in dark conditions, and this severely attenuates reliable light-controlled gene expression. Therefore, it is important to develop reliable PA-Gal4 transcription factors with robust light-induced gene expression and limited background activity. By optimization of synthetic PA-Gal4 transcription factors, we have validated configurations of Gal4 DNA biding domain, transcription activat

SUBMITTER: Nagasaki SC 

PROVIDER: S-EPMC10721950 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature