DNA Carrier-Assisted Molecular Ping-Pong in an Asymmetric Nanopore.

ABSTRACT: Nanopore analysis relies on ensemble averaging of translocation signals obtained from numerous molecules, requiring a relatively high sample concentration and a long turnaround time from the sample to results. The recapture and subsequent re-reading of the same molecule is a promising alternative that enriches the signal information from a single molecule. Here, we describe how an asymmetric nanopore improves molecular ping-pong by promoting the recapture of the molecule in the trans reservoir. We also demonstrate that the molecular recapture could be improved by linking the target molecule to a long DNA carrier to reduce the diffusion, thereby achieving over 100 recapture events. Using this ping-pong methodology, we demonstrate its use in accurately resolving nanostructure motifs along a

SUBMITTER: Zheng F 

PROVIDER: S-EPMC10722531 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature