Dataset Information

Sodium salicylate ameliorates exercise-induced muscle damage in mice by inhibiting NF-kB signaling.

ABSTRACT:

Background

Eccentric muscle contraction can cause muscle damage, which reduces the efficiency of exercise. Previous evidence suggested that Sodium salicylate (SS) could improve the repair of aged muscle. This study intends to investigate whether SS can impact skeletal muscle damage caused by eccentric exercise.

Methods

Eccentric treadmill exercise was performed to induce muscle damage in mice. Plasma levels of muscle damage markers were estimated. RT-qPCR was employed for detecting mRNA levels of proinflammatory mediators in murine gastrocnemius muscle. Immunofluorescence staining of laminin/DAPI was utilized for quantifying centrally nucleated myofibers in the gastrocnemius muscle. Western blotting was implemented to examine protein levels of mitsugumin 53 (MG53), matrix metalloproteinase (MMP)-2/9, and NF-κB signaling-related markers.

Results

SS administration reduced muscle damage marker production in the plasma and decreased the levels of proinflammatory mediators, MG53 and MMP-2/9 in mice after exercise. SS alleviated the severity of muscle damage in the gastrocnemius of mice after eccentric exercise. SS blocked NF-κB signaling pathway in the gastrocnemius muscle.

Conclusion

SS administration ameliorates skeletal muscle damage caused by eccentric exercise in the mouse model.

SUBMITTER: Wang Y

PROVIDER: S-EPMC10722820 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Publications

Sodium salicylate ameliorates exercise-induced muscle damage in mice by inhibiting NF-kB signaling.

Wang Yiming Y Sun Yuning Y Yang Chunhui C Han Bing B Wang Sining S

Journal of orthopaedic surgery and research 20231215 1

<h4>Background</h4>Eccentric muscle contraction can cause muscle damage, which reduces the efficiency of exercise. Previous evidence suggested that Sodium salicylate (SS) could improve the repair of aged muscle. This study intends to investigate whether SS can impact skeletal muscle damage caused by eccentric exercise.<h4>Methods</h4>Eccentric treadmill exercise was performed to induce muscle damage in mice. Plasma levels of muscle damage markers were estimated. RT-qPCR was employed for detectin ...[more]

PMID: 38098039

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Dataset Information

Sodium salicylate ameliorates exercise-induced muscle damage in mice by inhibiting NF-kB signaling.

Background

Methods

Results

Conclusion

Publications

Sodium salicylate ameliorates exercise-induced muscle damage in mice by inhibiting NF-kB signaling.

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