Three-dimensional histology reveals dissociable human hippocampal long axis gradients of Alzheimer's pathology.

ABSTRACT:

Introduction

Three-dimensional (3D) histology analyses are essential to overcome sampling variability and understand pathological differences beyond the dissection axis. We present Path2MR, the first pipeline allowing 3D reconstruction of sparse human histology without an MRI reference. We implemented Path2MR with post-mortem hippocampal sections to explore pathology gradients in Alzheimer's Disease.

Methods

Blockface photographs of brain hemisphere slices are used for 3D reconstruction, from which an MRI-like image is generated using machine learning. Histology sections are aligned to the reconstructed hemisphere and subsequently to an atlas in standard space.

Results

Path2MR successfully registered histological sections to their anatomical position along the hippoca

SUBMITTER: Ortega-Cruz D 

PROVIDER: S-EPMC10723286 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature