Mesenchymal Wnts are required for morphogenetic movements of calvarial osteoblasts during apical expansion.

ABSTRACT: Apical expansion of calvarial osteoblast progenitors from the cranial mesenchyme (CM) above the eye is integral for calvarial growth and enclosure of the brain. The cellular behaviors and signals underlying the morphogenetic process of calvarial expansion are unknown. During apical expansion, we found that mouse calvarial primordia have consistent cellular proliferation, density, and survival with complex tissue scale deformations, raising the possibility that morphogenetic movements underlie expansion. Time lapse light sheet imaging of mouse embryos revealed that calvarial progenitors intercalate in 3D to converge supraorbital arch mesenchyme mediolaterally and extend it apically. In contrast, progenitors located further apically exhibited protrusive and crawling activity. CM cells expres

SUBMITTER: Polsani N 

PROVIDER: S-EPMC10723314 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature