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Electrochemical DNA-based sensors for measuring cell-generated forces.

ABSTRACT: Mechanical forces play an important role in cellular communication and signaling. We developed in this study novel electrochemical DNA-based force sensors for measuring cell-generated adhesion forces. Two types of DNA probes, i.e., tension gauge tether and DNA hairpin, were constructed on the surface of a smartphone-based electrochemical device to detect piconewton-scale cellular forces at tunable levels. Upon experiencing cellular tension, the unfolding of DNA probes induces the separation of redox reporters from the surface of the electrode, which results in detectable electrochemical signals. Using integrin-mediated cell adhesion as an example, our results indicated that these electrochemical sensors can be used for highly sensitive, robust, simple, and portable measurement of cell-generated forces.

SUBMITTER: Tabrizi MA

PROVIDER: S-EPMC10723317 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Electrochemical DNA-based sensors for measuring cell-generated forces.

Tabrizi Mahmoud Amouzadeh MA Bhattacharyya Priyanka P Zheng Ru R You Mingxu M

bioRxiv : the preprint server for biology 20231205

Mechanical forces play an important role in cellular communication and signaling. We developed in this study novel electrochemical DNA-based force sensors for measuring cell-generated adhesion forces. Two types of DNA probes, i.e., tension gauge tether and DNA hairpin, were constructed on the surface of a smartphone-based electrochemical device to detect piconewton-scale cellular forces at tunable levels. Upon experiencing cellular tension, the unfolding of DNA probes induces the separation of r ...[more]

PMID: 38106148

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Electrochemical DNA-based sensors for measuring cell-generated forces.

Publications

Electrochemical DNA-based sensors for measuring cell-generated forces.

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