Temporal Tracking of Plasma Cells in vivo Using J-chain CreERT2 Reporter System.

ABSTRACT: Plasma cells (PCs) are essential for humoral immunity, as they are responsible for the production of antibodies and contribute to immunological memory. Despite their importance, differentiating between long-lived and short-lived PCs in vivo remains a challenge due to a lack of specific markers to distinguish these populations. Addressing this gap, our study introduces a novel J-chain CreERT2 GFP allele (IgJ^CreERT2) for precise genetic studies of PCs. This model takes advantage of PC-restricted expression of the J-chain gene, enabling temporal and cell-specific tracking of PCs utilizing a tamoxifen-inducible Cre recombinase. Our in vitro and in vivo validation studies of the inducible Cre allele confirmed the fidelity and utility of this model and demonstrate

SUBMITTER: Borbet TC 

PROVIDER: S-EPMC10723324 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature