Permanent deconstruction of intracellular primary cilia in differentiating granule cell neurons.

ABSTRACT: Primary cilia on granule cell neuron progenitors in the developing cerebellum detect sonic hedgehog to facilitate proliferation. Following differentiation, cerebellar granule cells become the most abundant neuronal cell type in the brain. While essential during early developmental stages, the fate of granule cell cilia is unknown. Here, we provide nanoscopic resolution of ciliary dynamics in situ by studying developmental changes in granule cell cilia using large-scale electron microscopy volumes and immunostaining of mouse cerebella. We found that many granule cell primary cilia were intracellular and concealed from the external environment. Cilia were disassembed in differentiating granule cell neurons in a process we call cilia deconstruction that was distinct from pre-mitotic ci

SUBMITTER: Ott CM 

PROVIDER: S-EPMC10723395 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature