Quantitative Dynamic Analysis of de novo Sphingolipid Biosynthesis in Arabidopsis thaliana.

ABSTRACT: Sphingolipids are pivotal for plant development and stress responses. Growing interest has been directed towards fully comprehending the regulatory mechanisms of the sphingolipid pathway. We explore its de novo biosynthesis and homeostasis in Arabidopsis thaliana cell cultures, shedding light on fundamental metabolic mechanisms. Employing ¹⁵N isotope labeling and quantitative dynamic modeling approach, we developed a regularized and constraint-based Dynamic Metabolic Flux Analysis (r-DMFA) framework to predict metabolic shifts due to enzymatic changes. Our analysis revealed key enzymes such as sphingoid-base hydroxylase (SBH) and long-chain-base kinase (LCBK) to be critical for maintaining sphingolipid homeostasis. Disruptions in

SUBMITTER: Osinuga A 

PROVIDER: S-EPMC10723408 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature