Multi-omics analyses reveal novel effects of PLCγ2 deficiency in the mouse brain.

ABSTRACT: Phospholipase C gamma-2 (PLCγ2) catalyzes the hydrolysis of the membrane phosphatidylinositol-4,5-bisphosphate (PIP₂) to form diacylglycerol (DAG) and inositol trisphosphate (IP₃), which subsequently feed into numerous downstream signaling pathways. PLCG2 polymorphisms are associated with both reduced and increased risk of Alzheimer's disease (AD) and with longevity. In the brain, PLCG2 is highly expressed in microglia, where it is proposed to regulate phagocytosis, secretion of cytokines/chemokines, cell survival and proliferation. We analyzed the brains of three-month-old PLCγ2 knockout (KO), heterozygous (HET), and wild-type (WT) mice using multiomics approaches, including shotgun lipidomics, proteomics, and gene expression profiling, and immunofluorescence. Lipido

SUBMITTER: Hopp SC 

PROVIDER: S-EPMC10723468 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature