Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer.

ABSTRACT:

Background

There are important unmet clinical needs to develop cell enrichment technologies to enable unbiased label-free isolation of both single cell and clusters of circulating tumor cells (CTCs) manifesting heterogeneous lineage specificity. Here, we report a pilot study based on microfluidic acoustophoresis enrichment of CTCs using the CellSearch CTC assay as a reference modality.

Methods

Acoustophoresis uses an ultrasonic standing wave field to separate cells based on biomechanical properties (size, density, and compressibility) resulting in inherently label-free and epitope-independent cell enrichment. Following red blood cell lysis and paraformaldehyde fixation, 6 mL of whole blood from 12 patients with metastatic prostate cancer and 20 healthy controls were processe

SUBMITTER: Magnusson C 

PROVIDER: S-EPMC10723509 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature