Association of elevated autoantibody to high expression of GNAS in hepatocellular carcinoma.

ABSTRACT:

Purpose

This study was based on hepatocellular carcinoma (HCC) patients of early-stage to explore the diagnostic capability and possible production causes of anti-GNAS autoantibody.

Methods

We evaluated the frequency of anti-GNAS autoantibody in sera from patients with early-stage HCC by enzyme-linked immunosorbent assay (ELISA) and the expression of GNAS protein in early-stage HCC tissues by immunohistochemistry. Western blotting (WB) and real-time polymerase chain reaction (RT-PCR) were utilized to examine the expressions of GNAS protein and mRNA in cell lines. GEO and International Cancer Genome Consortium (ICGC) databases were inquired to explore mRNA expression and mutation of GNAS in HCC tissues.

Results

The positive rates of anti-GNAS autoantibody in HCC patien

SUBMITTER: Wang K 

PROVIDER: S-EPMC10724561 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature