Validation of method for faecal sampling in cats and dogs for faecal microbiome analysis.

ABSTRACT:

Background

Reproducible and reliable studies of cat and dog faecal microbiomes are dependent on many methodology-based variables including how the faecal stools are sampled and stored prior to processing. The current study aimed to establish an appropriate method for sampling and storing faecal stools from cats and dogs which may also be applied to privately-owned pets. The approach investigated the effects of storing faeces for up to 12 h at room temperature and sampling from various locations within the stool in terms of microbial diversity, relative taxa abundances and DNA yield. Faeces were collected from 10 healthy cats and 10 healthy dogs and stored at room temperature (20 °C). Samples were taken from various locations within the stool (the first emitted part (i), the middle

SUBMITTER: Langon X 

PROVIDER: S-EPMC10724939 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature