Plaque Microbiome in Caries-Active and Caries-Free Teeth by Dentition.

ABSTRACT:

Objective

Describe associations between dental caries and dental plaque microbiome, by dentition and family membership.

Methods

This cross-sectional analysis included 584 participants in the Center for Oral Health Research in Appalachia Cohort 1 (COHRA1). We sequenced the 16S ribosomal RNA gene (V4 region) of frozen supragingival plaque, collected 10 y prior, from 185 caries-active (enamel and dentinal) and 565 caries-free (no lesions) teeth using the Illumina MiSeq platform. Sequences were filtered using the R DADA2 package and assigned taxonomy using the Human Oral Microbiome Database.

Results

Microbiomes of caries-active and caries-free teeth were most similar in primary dentition and least similar in permanent dentition, but caries-active teeth were significantly

SUBMITTER: Bhaumik D 

PROVIDER: S-EPMC10725180 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature