Digital PCR to Measure SARS-CoV-2 RNA, Variants, and Outcomes in Youth.

ABSTRACT:

Background

The role of SARS-CoV-2 viral load in predicting contagiousness, disease severity, transmissibility, and clinical decision-making continues to be an area of great interest. However, most studies have been in adults and have evaluated SARS-CoV-2 loads using cycle thresholds (Ct) values, which are not standardized preventing consistent interpretation critical to understanding clinical impact and utility. Here, a quantitative SARS-CoV-2 reverse-transcription digital PCR (RT-dPCR) assay normalized to WHO International Units was applied to children at risk of severe disease diagnosed with COVID-19 at St. Jude Children's Research Hospital between March 28, 2020, and January 31, 2022.

Methods

Demographic and clinical information from children, adolescents, and young adult

SUBMITTER: Hijano DR 

PROVIDER: S-EPMC10725239 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature