Experimental insights into electrocatalytic [Cp*Rh(bpy)Cl]⁺ mediated NADH regeneration.

ABSTRACT: NADH plays a crucial role in many enzymatically catalysed reactions. Due to the high costs of NADH a regeneration mechanism of this cofactor can enlarge the applications of enzymatic reactions dramatically. This paper gives a thorough system analysis of the mediated electrochemical regeneration of active NADH using cyclic voltammograms and potentiostatic measurements with varying pH, electrode potential, and electrolyte solution, highlighting the system's limiting conditions, elucidating optimal working parameters for the electrochemical reduction of NAD⁺, and bringing new insight on the oxidation of inactive reduction products. Using [Cp*Rh(bpy)Cl]⁺ as an electron mediator dramatically increases the percentage of enzymatically active electrochemically reduced NADH fr

SUBMITTER: Meyer J 

PROVIDER: S-EPMC10725497 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature