Characterisation of a Novel Hybrid IncFII_pHN7A8:IncR:IncN Plasmid Co-Harboring bla_NDM-5 and bla_KPC-2 from a Clinical ST11 Carbapenem-Resistant Klebsiella pneumoniae Strain.

ABSTRACT:

Purpose

We aimed to characterize a novel bla_NDM-5 and bla_KPC-2 co-carrying hybrid plasmid from a clinical carbapenem-resistant Klebsiella pneumoniae (CRKP) strain.

Methods

Antimicrobial susceptibility was determined by the broth microdilution method. Plasmid size and localization were estimated using S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting. Plasmid transfer ability was evaluated by conjugation experiments. Whole genome sequencing (WGS) was performed using Illumina NovaSeq6000 and Oxford Nanopore MinION platforms. Genomic characteristics were analyzed using bioinformatics methods.

Results

Strain ZY27320 was a multidrug-resistant (MDR) clinical ST11 K. pneumoniae strain that confers h

SUBMITTER: Sun L 

PROVIDER: S-EPMC10725640 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature