Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging.

ABSTRACT: The retroviral Gag precursor plays a central role in the selection and packaging of viral genomic RNA (gRNA) by binding to virus-specific packaging signal(s) (psi or ψ). Previously, we mapped the feline immunodeficiency virus (FIV) ψ to two discontinuous regions within the 5' end of the gRNA that assumes a higher order structure harboring several structural motifs. To better define the region and structural elements important for gRNA packaging, we methodically investigated these FIV ψ sequences using genetic, biochemical, and structure-function relationship approaches. Our mutational analysis revealed that the unpaired U⁸⁵CUG⁸⁸ stretch within FIV ψ is crucial for gRNA encapsidation into nascent virions. High-throughput selective 2' hydroxyl acylation analyzed by prim

SUBMITTER: Krishnan A 

PROVIDER: S-EPMC10726167 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature