On a roll: a direct comparison of extraction methods for the recovery of eDNA from roller swabbing of surfaces.

ABSTRACT:

Objective

Roller swabbing of surfaces is an effective way to obtain environmental DNA, but the current DNA extraction method for these samples is equipment heavy, time consuming, and increases potential contamination through multiple handling. Here, we used rollers to swab a dog kennel and compared three DNA extraction approaches (water filtration, roller trimming and direct buffer) using two different platforms (Qiacube, Kingfisher). DNA extraction methods were evaluated based on cost, effort, DNA concentration and PCR result.

Results

The roller trim method emerged as the optimal method with the best PCR results, DNA concentration and cost efficiency, while the buffer-based methods were the least labour intensive but produced mediocre PCR results and DNA concentrations. Add

SUBMITTER: Guthrie AM 

PROVIDER: S-EPMC10726604 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature