Lens epithelium cell ferroptosis mediated by m⁶A-lncRNA and GPX4 expression in lens tissue of age-related cataract.

ABSTRACT:

Background

In the present study, we explored the role of N⁶-methyladenosine (m⁶A) modification of long non-coding RNAs (lncRNAs) and its association with ferroptosis in lens epithelium cells (LECs) of age-related cataract (ARC).

Methods

Through m⁶A RNA immunoprecipitation sequencing (m⁶A-RIP-seq) and RNA sequencing (RNA-seq), we identified m⁶A mediated and differentially expressed lncRNAs (dme-lncRNAs) in ARC patients. Based on bioinformatics analysis, we selected critical dme-lncRNAs and pathways associated with ARC formation to reveal their potential molecular mechanisms. The downregulation of glutathione peroxidase 4 (GPX4), a key component of ferroptosis, was confirmed by real-time RT-PCR (RT-qPCR) and Western blotti

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10726616 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature