Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9.

ABSTRACT: Mammalian cell surface and secreted glycoproteins exhibit remarkable glycan structural diversity that contributes to numerous physiological and pathogenic interactions. Terminal glycan structures include Lewis antigens synthesized by a collection of α1,3/4-fucosyltransferases (CAZy GT10 family). At present, the only available crystallographic structure of a GT10 member is that of the Helicobacter pylori α1,3-fucosyltransferase, but mammalian GT10 fucosyltransferases are distinct in sequence and substrate specificity compared with the bacterial enzyme. Here, we determined crystal structures of human FUT9, an α1,3-fucosyltransferase that generates Lewis^x and Lewis^y antigens, in complex with GDP, acceptor glycans, and as a FUT9-donor analog-acceptor Michaelis complex. Th

SUBMITTER: Kadirvelraj R 

PROVIDER: S-EPMC10726971 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature