GARP and EARP are required for efficient BoHV-1 replication as identified by a genome wide CRISPR knockout screen.

ABSTRACT: The advances in gene editing bring unprecedented opportunities in high throughput functional genomics to animal research. Here we describe a genome wide CRISPR knockout library, btCRISPRko.v1, targeting all protein coding genes in the cattle genome. Using it, we conducted genome wide screens during Bovine Herpes Virus type 1 (BoHV-1) replication and compiled a list of pro-viral and anti-viral candidates. These candidates might influence multiple aspects of BoHV-1 biology such as viral entry, genome replication and transcription, viral protein trafficking and virion maturation in the cytoplasm. Some of the most intriguing examples are VPS51, VPS52 and VPS53 that code for subunits of two membrane tethering complexes, the endosome-associated recycling protein (EARP) complex and the Golgi-asso

SUBMITTER: Tan WS 

PROVIDER: S-EPMC10727446 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature