TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in Arabidopsis.

ABSTRACT: Proximity labeling was recently developed to detect protein-protein interactions and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (bait protein) to biotinylate adjacent proteins. The biotinylated protein can be purified by streptavidin beads, and identified by mass spectrometry (MS). TurboID is an engineered biotin ligase with high catalytic efficiency, which is used for proximity labeling. Although TurboID-based proximity labeling technology has been successfully established in mammals, its application in plant systems is limited. Here, we report the usage of TurboID for proximity labeling of FIP37, a core member of m⁶A methy

SUBMITTER: Li X 

PROVIDER: S-EPMC10727772 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature