S9.6-based hybrid capture immunoassay for pathogen detection.

ABSTRACT: The detection of pathogens is critical for clinical diagnosis and public health surveillance. Detection is usually done with nucleic acid-based tests (NATs) and rapid antigen tests (e.g., lateral flow assays [LFAs]). Although NATs are more sensitive and specific, their use is often limited in resource-poor settings due to specialized requirements. To address this limitation, we developed a rapid DNA-RNA Hybrid Capture immunoassay (HC) that specifically detects RNA from pathogens. This assay utilizes a unique monoclonal antibody, S9.6, which binds DNA-RNA hybrids. Biotinylated single-stranded DNA probes are hybridized to target RNAs, followed by hybrid capture on streptavidin and detection with S9.6. The HC-ELISA assay can detect as few as 10⁴ RNA molecules that are 2.2 kb in len

SUBMITTER: Bothra A 

PROVIDER: S-EPMC10728093 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature