Structure and dynamics of endogenous cardiac troponin complex in human heart tissue captured by native nanoproteomics.

ABSTRACT: Protein complexes are highly dynamic entities that display substantial diversity in their assembly, post-translational modifications, and non-covalent interactions, allowing them to play critical roles in various biological processes. The heterogeneity, dynamic nature, and low abundance of protein complexes in their native states present challenges to study using conventional structural biology techniques. Here we develop a native nanoproteomics strategy for the enrichment and subsequent native top-down mass spectrometry (nTDMS) analysis of endogenous cardiac troponin (cTn) complex directly from human heart tissue. The cTn complex is enriched and purified using peptide-functionalized superparamagnetic nanoparticles under non-denaturing conditions to enable the isotopic resolution of cTn co

SUBMITTER: Chapman EA 

PROVIDER: S-EPMC10728164 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature