Dataset Information

Lung microbiome alterations in patients with anti-Jo1 antisynthetase syndrome and interstitial lung disease.

ABSTRACT:

Aim

To characterize the lung microbiome in the bronchoalveolar lavage fluid (BALF) of patients with Antisynthetase Syndrome (ASSD) according to anti-Jo1 autoantibody positivity and evaluate the correlation with differential cell count and other bacterial genera in BALF.

Methods

We sequenced the 16S ribosomal RNA gene in the BALF of anti-Jo1-positive (JoP, n=6) and non-Jo1-positive (NJo, n=17) patients, and the differential cell count in BALF was evaluated. The Spearman's correlation was calculated for the quantitative variables and abundance of bacterial species.

Results

The Veillonella genus showed a significant decrease (p<0.01) in JoP (2.2%) in comparison to NJo (4.1%) patients. The correlation analysis showed several high (rho ≥ ± 0.7) and significant (p < 0.05) correlations. We analyzed the results obtained for the Veillonella genera and other study variables. The JoP group showed that the abundance of Veillonella had a high negative correlation with macrophages (rho = - 0.77) and a positive correlation with eosinophils (rho = 0.77), lymphocytes (rho = 0.77), and Prevotella (rho = 1).

Conclusions

The lung microbiome in ASSD patients differs and may affect cell composition, contributing to lung damage mechanisms. The presence of anti-Jo1 autoantibodies showed a low abundance of Veillonella. This genus had a strong and positive correlation with Prevotella abundance and levels of eosinophils and lymphocytes, and it showed a strong negative correlation with the percentage of macrophages.

SUBMITTER: Quintero-Puerta T

PROVIDER: S-EPMC10728596 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Lung microbiome alterations in patients with anti-Jo1 antisynthetase syndrome and interstitial lung disease.

Quintero-Puerta Teresa T Lira-Lucio Juan Alberto JA Falfán-Valencia Ramcés R Vega-Sánchez Ángel E ÁE Márquez-García Eduardo E Mejía Mayra M Bautista-Becerril Brandon B Rojas-Serrano Jorge J Ramos-Martínez Espiridión E Buendía-Roldán Ivette I Pérez-Rubio Gloria G

Frontiers in cellular and infection microbiology 20231205

<h4>Aim</h4>To characterize the lung microbiome in the bronchoalveolar lavage fluid (BALF) of patients with Antisynthetase Syndrome (ASSD) according to anti-Jo1 autoantibody positivity and evaluate the correlation with differential cell count and other bacterial genera in BALF.<h4>Methods</h4>We sequenced the 16S ribosomal RNA gene in the BALF of anti-Jo1-positive (JoP, n=6) and non-Jo1-positive (NJo, n=17) patients, and the differential cell count in BALF was evaluated. The Spearman's correlati ...[more]

PMID: 38116136

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Dataset Information

Lung microbiome alterations in patients with anti-Jo1 antisynthetase syndrome and interstitial lung disease.

Aim

Methods

Results

Conclusions

Publications

Lung microbiome alterations in patients with anti-Jo1 antisynthetase syndrome and interstitial lung disease.

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