Genotype analysis to clarify RhD variants in discrepant samples of Chilean population.

ABSTRACT:

Introduction

The D antigen variants are classified as weak, partial, and extremely weak (DEL) and can be differentiated using molecular tests. In Chile, the laboratories of local blood centers do not identify variants of the D antigen, referring them for study to the Reference Laboratory of the Public Health Institute of Chile. So, our aim was to talk about the results of the molecular analysis of variants of the D antigen in samples that had different results in the serological classification.

Methods

In the D antigen classification of the Rh system, 479 samples with serological discrepant results were sent for molecular analysis. The Rh phenotype was performed with monoclonal anti-C, anti-c, anti-E, and anti-e antisera by direct agglutination. To find the D antigen, resear

SUBMITTER: Aburto A 

PROVIDER: S-EPMC10728993 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature