PD-L1 expression levels in mesenchymal stromal cells predict their therapeutic values for autoimmune hepatitis.

ABSTRACT:

Background

Autoimmune hepatitis is a chronic inflammatory hepatic disorder with no effective treatment. Mesenchymal stromal cells (MSCs) have emerged as a promising treatment owing to their unique advantages. However, their heterogeneity is hampering use in clinical applications.

Methods

Wharton's jelly derived MSCs (WJ-MSCs) were isolated from 58 human donors using current good manufacturing practice conditions. Gene expression profiles of the WJ-MSCs were analyzed by transcriptome and single-cell RNA-sequencing (scRNA-seq), and subsequent functional differences were assessed. Expression levels of programmed death-ligand 1 (PD-L1) were used as an indicator to screen WJ-MSCs with varied immunomodulation activities and assessed their corresponding therapeutic effects in a mou

SUBMITTER: Bai X 

PROVIDER: S-EPMC10729378 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature