Performance evaluation of protocols for Taenia saginata and Ascaris suum egg recovery from the house fly's gastrointestinal tract and exoskeleton.

ABSTRACT:

Background

The synanthropic house fly (Musca domestica) can potentially contribute to the mechanical spread of eggs of Taenia and Ascaris spp. in the environment and between hosts. However, the absence of validated protocols to recover eggs hampers an in-depth analysis of the house fly's role in parasite egg transmission.

Methods

The gastrointestinal tract and exoskeleton of euthanized house flies were spiked with Taenia saginata eggs. The performance of several recovery protocols, in terms of both the recovery rate and ease-of-use, was (microscopically) evaluated and compared. These protocols employed steps such as washing, maceration, filtration, flotation and both passive and centrifugal sedimentation. The final validated protocols were subsequently evaluated for the reco

SUBMITTER: De Bock S 

PROVIDER: S-EPMC10729472 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature