Four ways of implementing robustness quantification in strain characterisation.

ABSTRACT:

Background

In industrial bioprocesses, microorganisms are generally selected based on performance, whereas robustness, i.e., the ability of a system to maintain a stable performance, has been overlooked due to the challenges in its quantification and implementation into routine experimental procedures. This work presents four ways of implementing robustness quantification during strain characterisation. One Saccharomyces cerevisiae laboratory strain (CEN.PK113-7D) and two industrial strains (Ethanol Red and PE2) grown in seven different lignocellulosic hydrolysates were assessed for growth-related functions (specific growth rate, product yields, etc.) and eight intracellular parameters (using fluorescent biosensors).

Results

Using flasks and high-throughput experimental setu

SUBMITTER: Torello Pianale L 

PROVIDER: S-EPMC10729505 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature