Environmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan.

ABSTRACT:

Background

Mycetoma is a chronic disease affecting the skin and subcutaneous tissue endemic in the tropical and subtropical regions. Several bacteria and fungi can cause mycetoma, but fungal mycetoma (eumycetoma) is challenging because the treatment requires a combination of a long-term antifungal agent and surgery. Although the transmission route has not yet been elucidated, infection from the soil is a leading hypothesis. However, there are few soil investigation studies, and the geographical distribution of mycetoma pathogens is not well documented. Here, we used multiplex real-time PCR technology to identify three fungal species from soil samples.

Methods

In total, 64 DNA samples were extracted from soil collected in seven villages in an endemic area in Sennar State, Sud

SUBMITTER: Hashizume H 

PROVIDER: S-EPMC10729560 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature