Mesenchymal stem cells lose the senescent phenotype under 3D cultivation.

ABSTRACT:

Background

Three-dimensional (3D) cell culture is widely used in various fields of cell biology. In comparison to conventional two-dimensional (2D) cell culture, 3D cell culture facilitates a more accurate replication of the in vivo microenvironment, which is essential for obtaining more relevant results. The application of 3D cell culture techniques in regenerative medicine, particularly in mesenchymal stem cell (MSC)-based research, has been extensively studied. Many of these studies focus on the enhanced paracrine activity of MSCs cultured in 3D environments. However, few focus on the cellular processes that occur during 3D cultivation.

Methods

In this work, we studied the changes occurring within 3D-cultured MSCs (3D-MSCs). Specifically, we examined the expression of num

SUBMITTER: Krasnova O 

PROVIDER: S-EPMC10729581 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature