Development of novel isobaric tags enables accurate and sensitive multiplexed proteomics using complementary ions.

ABSTRACT: High-throughput quantitative analysis of the cells' proteomes across multiple conditions such as various perturbations and different time points is essential for gaining insights into treatment-induced biological responses or disease pathological states. The advancements in mass spectrometry instrumentation and isobaric labeling methods provided useful tools to help address such demands. However, the current widely adopted isobaric labeling methods such as tandem mass tag (TMT) and isobaric tags for relative and absolute quantitation (iTRAQ) are based on low-mass reporter ions, which are indistinguishable among different peptide analytes, to achieve relative quantification. Therefore, these methods intrinsically suffer from severe ratio distortion when analyzing complex samples due to pept

SUBMITTER: Li M 

PROVIDER: S-EPMC10729713 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature