Standardization of Fluorescent Reporter Assays in Synthetic Biology across the Visible Light Spectrum.

ABSTRACT: In synthetic biology, Fluorescent reporters are frequently used to characterize the expression levels obtained from both genetic parts such as promoters and ribosome binding sites as well as from complex genetic circuits. To this end, plate readers offer an easy and high-throughput way of characterizing both the growth and fluorescence expression levels of cell cultures. However, despite the similar mode of action used in different devices, their output is not comparable due to intrinsic differences in their setup. Additionally, the generated output is expressed using arbitrary units, limiting reliable comparison of results to measurements taken within one single experiment using one specific plate reader, hampering the transferability of data across different plate readers and laboratorie

SUBMITTER: De Wannemaeker L 

PROVIDER: S-EPMC10729763 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature