Heparin-binding epidermal growth factor and fibroblast growth factor 2 rescue Muller glia-derived progenitor cell formation in microglia- and macrophage-ablated chick retinas.

ABSTRACT: Recent studies have demonstrated the impact of pro-inflammatory signaling and reactive microglia/macrophages on the formation of Müller glial-derived progenitor cells (MGPCs) in the retina. In chick retina, ablation of microglia/macrophages prevents the formation of MGPCs. Analyses of single-cell RNA-sequencing chick retinal libraries revealed that quiescent and activated microglia/macrophages have a significant impact upon the transcriptomic profile of Müller glia (MG). In damaged monocyte-depleted retinas, MG fail to upregulate genes related to different cell signaling pathways, including those related to Wnt, heparin-binding epidermal growth factor (HBEGF), fibroblast growth factor (FGF) and retinoic acid receptors. Inhibition of GSK3β, to simulate Wnt signaling, failed to rescue the de

SUBMITTER: El-Hodiri HM 

PROVIDER: S-EPMC10730090 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature