Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?

ABSTRACT:

Introduction

Enterovirus E (EV-E) is a common viral pathogen endemic in cattle worldwide. Little is known, however, about its potential interactions with bovine immune cells.

Material and methods

The EV-E-permissiveness of bovine peripheral blood mononuclear cells (PBMCs) was evaluated. The infectious titres of extracellular virus were measured and the intracellular viral RNA levels were determined by reverse transcription quantitative PCR after cell inoculation. The effects of EV-E on cell viability and proliferative response were investigated with a methyl thiazolyl tetrazolium bromide reduction assay, the percentages of main lymphocyte subsets and oxidative burst activity of blood phagocytes were determined with flow cytometry, and pro-inflammatory cytokine secretion was

SUBMITTER: Malaczewska J 

PROVIDER: S-EPMC10730555 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature