Functional characterization of three G protein-coupled acetylcholine receptors in parasitic nematode Trichinella spiralis.

ABSTRACT: The physiological significance of metabotropic acetylcholine receptors in parasitic nematodes remains largely unexplored. Here, three different Trichinella spiralis G protein-coupled acetylcholine receptors (TsGAR-1, -2, and -3) were identified in the genome of T. spiralis. The phylogenetic analyses showed that TsGAR-1 and -2 receptors belong to a distinct clade specific to invertebrates, while TsGAR-3 is closest to the cluster of mammalian-type muscarinic acetylcholine receptors (mAChR). The mRNA of TsGAR-1, -2, and -3 was detected in muscle larvae, newborn larvae, and adults. The functional aequorin-based assay in Chinese hamster ovary cells revealed that all three types of T. spiralis GARs trigger the G_q/11 pathway upon activation of the receptor with the acetylcholine ligand

SUBMITTER: Ning C 

PROVIDER: S-EPMC10731000 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature