Engineered pine endophytic Bacillus toyonensis with nematocidal and colonization abilities for pine wilt disease control.

ABSTRACT:

Introduction

The pinewood nematode (PWN) is responsible for causing pine wilt disease (PWD), which has led to the significant decline of conifer species in Eurasian forests and has become a globally invasive quarantine pest. Manipulating plant-associated microbes to control nematodes is an important strategy for sustainable pest management. However, it has proven difficult to find pine-associated bacteria that possess both nematocidal activity and the ability to colonize pine tissues.

Methods

The stress experiments with turpentine and pine tissue extract were carried out to screen for the desired target strain that could adapt to the internal environment of pine trees. This strain was used to construct an engineered nematocidal strain. Additionally, a fluorescent strain was

SUBMITTER: Li D 

PROVIDER: S-EPMC10731049 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature