Single-cell and spatial transcriptomics reveal POSTN⁺ cancer-associated fibroblasts correlated with immune suppression and tumour progression in non-small cell lung cancer.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) are potential targets for cancer therapy. Due to the heterogeneity of CAFs, the influence of CAF subpopulations on the progression of lung cancer is still unclear, which impedes the translational advances in targeting CAFs.

Methods

We performed single-cell RNA sequencing (scRNA-seq) on tumour, paired tumour-adjacent, and normal samples from 16 non-small cell lung cancer (NSCLC) patients. CAF subpopulations were analyzed after integration with published NSCLC scRNA-seq data. SpaTial enhanced resolution omics-sequencing (Stereo-seq) was applied in tumour and tumour-adjacent samples from seven NSCLC patients to map the architecture of major cell populations in tumour microenvironment (TME). Immunohistochemistry (IHC) and multiplex

SUBMITTER: Chen C 

PROVIDER: S-EPMC10731139 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature