Increased Osteoblast Gα_S Promotes Ossification by Suppressing Cartilage and Enhancing Callus Mineralization During Fracture Repair in Mice.

ABSTRACT: Gα_S, the stimulatory G protein α-subunit that raises intracellular cAMP levels by activating adenylyl cyclase, plays a vital role in bone development, maintenance, and remodeling. Previously, using transgenic mice overexpressing Gα_S in osteoblasts (G_S-Tg), we demonstrated the influence of osteoblast Gα_S level on osteogenesis, bone turnover, and skeletal responses to hyperparathyroidism. To further investigate whether alterations in Gα_S levels affect endochondral bone repair, a postnatal bone regenerative process that recapitulates embryonic bone development, we performed stabilized tibial osteotomy in male G_S-Tg mice at 8 weeks of age and examined the progression of fracture healing by micro-CT, histomorphometry, and gene ex

SUBMITTER: Lee KK 

PROVIDER: S-EPMC10731140 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature